abstract：:The ability of several metals to inhibit dopamine beta-monooxygenase was measured and compared with their ability to compete with the binding of 64Cu to the water-soluble form of the bovine adrenal enzyme at pH 6.0. In the presence of an optimal concentration of copper (0.5 microM in the present assay system), an inhi...

journal_title：Journal of inorganic biochemistry

authors： Skotland T,Flatmark T

更新日期：1984-01-01 00:00:00

abstract：:Measurements of binding of certain divalent cations to yeast apoenolase were made using a pH-meter, chromatography, a divalent cation electrode, and ultrafiltration. The binding of the activating metal ions Mg2+ and Co2+ and the nonactivator Ca2+ were studied as functions of the presence or absence of substrate/produc...

journal_title：Journal of inorganic biochemistry

authors： Brewer JM,Carreira LA,Collins KM,Duvall MC,Cohen C,DerVartanian DV

更新日期：1983-11-01 00:00:00

abstract：:The results of 1H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together wi...

journal_title：Journal of inorganic biochemistry

authors： Otiko G,Razi MT,Sadler PJ,Isab AA,Rabenstein DL

更新日期：1983-11-01 00:00:00

abstract：:Deferriferrioxamine B (H3DFB) is a linear trihydroxamic acid siderophore with molecular formula NH2(CH2)5[N(OH)C(O)(CH2)2C(O)NH(CH2)5]2N(OH)C(O)CH3 that forms a kinetically and thermodynamically stable complex with iron(III), ferrioxamine B. Under the conditions of our study (pH = 4.30, 25 degrees C), ferrioxamine B, ...

journal_title：Journal of inorganic biochemistry

authors： Monzyk B,Crumbliss AL

更新日期：1983-08-01 00:00:00

abstract：:The coordination structures of various species in the copper(II)-L-histidine (1:2) system in aqueous solution have been deduced by investigating the pH dependence of the electronic and circular dichroism spectra. The contribution to the spectra of the glycine-like and histamine-like binding modes of L-histidine has be...

journal_title：Journal of inorganic biochemistry

authors： Casella L,Gullotti M

更新日期：1983-02-01 00:00:00

abstract：:The kinetics of reduction of a peroxidatically active intermediate (formed by reaction of deuteroferriheme with hydrogen peroxide) by phenol and a series of substituted phenols have been studied by stopped-flow spectrophotometry. The complex effects of varying heme concentration have been interpreted in terms of the f...

journal_title：Journal of inorganic biochemistry

authors： Jones P,Mantle D,Wilson I

更新日期：1982-12-01 00:00:00

abstract：:Proton magnetic resonance spectroscopy has been employed to study the solution conformation of three cleavage fragments of troponin-C, each containing a single Ca(II)-binding site and corresponding to different regions in the primary sequence; viz. CB8 (residues 46-77), CB9 (residues 85-134) and TH2 (residues 121-159)...

journal_title：Journal of inorganic biochemistry

authors： Leavis PC,Evans JS,Levine BA

更新日期：1982-07-01 00:00:00

abstract：:Measurement and interpretation of the EXAFS associated with the K-absorption edge of zinc atoms in sheep liver metallothionein indicate that the primary coordination shell of each of these metal atoms comprises four sulphur atoms, with the Zn-S distance being 2.29 +/- 0.02 A. ...

journal_title：Journal of inorganic biochemistry

authors： Garner CD,Hasain SS,Bremner I,Bordas J

更新日期：1982-06-01 00:00:00

abstract：:Subcellular fractions were prepared by differential centrifugation from heart and liver of a lamb labeled with 75Se-selenite. Crude fractions of nuclei, mitochondria, and microsomes from both tissues were solubilized with sodium dodecyl sulfate and chromatographed on columns of sephacryl S-200. A low molecular weight ...

journal_title：Journal of inorganic biochemistry

authors： Beilstein MA,Tripp MJ,Whanger PD

更新日期：1981-12-01 00:00:00

abstract：:Antitumor antibiotic bleomycin can bind to and degrade DNA, both in vivo and in vitro. This DNA damaging property in vitro can be related to its ability to chelate ferrous ions under aerobic conditions leading to the formation of "active oxygens," which are thought to be directly responsible for the damage. At present...

journal_title：Journal of inorganic biochemistry

authors： Gutteridge JM,Shute DJ

更新日期：1981-12-01 00:00:00

abstract：:A recent and important approach to investigating electron transfer mechanisms of redox proteins has been through kinetic-ionic strength studies. There is, however, significant controversy as to whether such studies (1) yield information regarding the charge (or location) of the electron transfer site or (2) more simpl...

journal_title：Journal of inorganic biochemistry

authors： Feinberg BA,Ryan MD

更新日期：1981-11-01 00:00:00

abstract：:the reactions of chloroauric acid (HAuCl4) with inosine=ino, guanosine=guo, triacetylinosine=trino, triacetylguanosine=trguo, and cytidine=cyd were studied. Complexes of AU(III) and AU(I) with these nucleosides have been isolated from the reactions at different pH values in aqueous and in methanolic solutions. The Au(...

journal_title：Journal of inorganic biochemistry

authors： Hadjiliadis N,Pneumatikakis G,Basosi R

更新日期：1981-04-01 00:00:00

abstract：:We have studied the electron paramagnetic resonance (epr) spectra of complexes of apo-yeast enolase with 65Cu+2 in the presence and absence of substrate and magnesium ion. An unusual epr spectrum with large g parallel, large g and A rhombicity and very narrow line-widths (10 G) is seen for the first two 65Cu+2 bound i...

journal_title：Journal of inorganic biochemistry

authors： Dickinson LC,Rose SL,Westhead EW

更新日期：1980-12-01 00:00:00

abstract：:Cystine, selenocytsine, and several analogs were reduced by dithiothreitol (DTT), beta-mercaptoethanol (ME) and sodium borohydride (NaBH4). DTT was the most effective; DTT to cystine ratios from 10 to 80 were equally effective. With selenocysteine, however, absorption was considerably reduced at all ratios. Selenocyst...

journal_title：Journal of inorganic biochemistry

authors： Burnell JN,Karle JA,Shrift A

更新日期：1980-07-01 00:00:00

abstract：:The study of possible relationships between iron and vanadium metabolism (E. Sabbioni and E. Marafante, Proc. XIth Int. Conf. Biochem., 13-5-R122, Toronto, Canada) was extended to the vanadium in the biochemical mechanisms which involve the exchange of iron between transferrin and ferritin. The transfer of vanadium be...

journal_title：Journal of inorganic biochemistry

authors： Sabbioni E,Rade J,Bertolero F

更新日期：1980-07-01 00:00:00

abstract：:Complex formation between D-penicillamine (Pen) and copper(II) ions has been studied under simulated physiological conditions in both the presence and absence of the blood plasma constituents albumin, alanine, histidine, and zinc(II). Chromatographic and uv/vis and electron spin resonance (esr) spectroscopic methods w...

journal_title：Journal of inorganic biochemistry

authors： Laurie SH,Prime DM

更新日期：1979-11-01 00:00:00

abstract：:L-Cysteinatogold(I) was prepared by the reaction of L-cysteine with KAuBr4 in acidic media and its solubility determined from pH 4 to 10. The solubility at pH 7.4 and 37 degrees C is 1 microM. In the presence of excess cysteine, the solubility increases because of formation of bis(L-cysteinato)gold(I). The equilibrium...

journal_title：Journal of inorganic biochemistry

authors： Shaw CF 3rd,Schmitz G,Thompson HO,Witkiewicz P

更新日期：1979-07-01 00:00:00